| Abstract
|
<p>An automated peak picking strateg … <p>An automated peak picking strategy is presented where several peak sets with different signal-to-noise levels are combined to form a more reliable statement on the protein identity. The strategy is compared against both manual peak picking and industry standard automated peak picking on a set of mass spectra obtained after tryptic in gel digestion of 2D-gel samples from human fetal fibroblasts. The set of spectra contain samples ranging from strong to weak spectra, and the proposed multiple-scale method is shown to be much better on weak spectra than the industry standard method and a human operator, and equal in performance to these on strong and medium strong spectra. It is also demonstrated that peak sets selected by a human operator display a considerable variability and that it is impossible to speak of a single “true” peak set for a given spectrum. The described multiple-scale strategy both avoids time-consuming parameter tuning and exceeds the human operator in protein identification efficiency. The strategy therefore promises reliable automated user-independent protein identification using peptide mass fingerprints.</p>using peptide mass fingerprints.</p>
|
| Author
|
Thorsteinn Rögnvaldsson +
, Jari Häkkinen +
, Claes Lindberg +
, György Marko-Varga +
, Frank Potthast +
, Jim Samuelsson +
|
| DOI
|
http://dx.doi.org/10.1016/j.jchromb.2004.04.010 +
|
| Diva
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http://hh.diva-portal.org/smash/record.jsf?searchId=1&pid=diva2:237403
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| EndPage
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215 +
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| Issue
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2 +
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| Journal
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Journal of chromatography. B +
|
| PublicationType
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Journal Paper +
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| Publisher
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Elsevier +
|
| StartPage
|
209 +
|
| Title
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Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
|
| Volume
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807 +
|
| Year
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2004 +
|
| Has queryThis property is a special property in this wiki.
|
Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
, Publications:Improving automatic peptide mass fingerprint protein identification by combining many peak sets +
|
| Categories |
Publication +
|
| Modification dateThis property is a special property in this wiki.
|
30 September 2016 20:41:42 +
|
