| Abstract
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<p>Piums®, a new protein identificat … <p>Piums®, a new protein identification tool for peptide fingerprints, is presented. Piums includes both a peak extraction tool (Pepex®) and a new protein scoring algorithm (Piped®). The basic ideas underlying the scoring algorithm are presented and it is demonstrated on some real sample spectra. It is shown, using simulated peak lists, how the scoring performance varies with contamination levels and protein sequence coverage, and that there is a boundary for when scoring is possible. Piums is fully scriptable and modularised. Each individual module can be used by itself, with input and output in XML format, to e.g. include it in an analysis chain. Piums is benchmarked against the Mascot software from Matrix Science LLC, The results indicate that Piums is more precise than the Mascot score, and about 5-10% more efficient than Mascot for real peak lists, for the same level of false positives.</p>e same level of false positives.</p>
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| Author
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Jim Samuelsson +
, Daniel Dalevi +
, Rikard Aim +
, Thorsteinn Rögnvaldsson +
, Fránk Potthast +
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| Conference
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50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, Florida, United States, June 2-6, 2002
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| Diva
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http://hh.diva-portal.org/smash/record.jsf?searchId=1&pid=diva2:1392766
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| EndPage
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784 +
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| HostPublication
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Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics +
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| PublicationType
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Conference Paper +
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| Publisher
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ASMS +
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| StartPage
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783 +
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| Title
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PIUMS® - A new algorithm for protein identification using peptide fingerprints +
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| Year
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2002 +
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| Has queryThis property is a special property in this wiki.
|
Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
, Publications:PIUMS® - A new algorithm for protein identification using peptide fingerprints +
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| Categories |
Publication +
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| Modification dateThis property is a special property in this wiki.
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11 February 2020 21:22:14 +
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